Intestinal Adaptation Laboratory 

Murine Model for Small Bowel Resection

Suture of mouse small intestine.

The mouse, because of its small size, typically has presented a formidable technical challenge for safe resection and reanastomosis of the small bowel. With the advent of transgenic technology, the mouse is the ideal animal for characterization of adaptation following small bowel resection, because the expression of candidate factors considered essential may be genetically abolished or amplified. Further, transgenic models permit a means to understand temporal, spatial and stimulus-responsive regulation of gene expression. Our laboratory has perfected a murine model for small bowel resection with a resultant survival of greater than 80 percent.

Related Publications
Jarboe MD, Juno RJ, Stehr W, Bernal NP, Profitt S, Erwin CR, Warner BW. Epidermal growth factor receptor signaling regulates goblet cell production after small bowel resection. J Pediatr Surg, 40(1): 92-97, 2005.

Bernal NP, Stehr W, Zhang Y, Profitt P, Erwin CR, Warner BW. Evidence for active wnt signaling during postresection intestinal adaptation. J Pediatr Surg, 40(6): 1025-1029, 2005.

Stehr W, Mercer TI, Bernal NP, Erwin CR, Warner BW. Opposing Roles for p21waf1/cip1 and p27kip1 in Enterocyte Differentiation, Proliferation, and Migration. Surgery, 138(2): 187-94, 2005.

Bernal NP, Stehr W, Profitt P Erwin CR, Warner BW. Combined pharmacotherapy that increases proliferation and decreases apoptosis optimally enhances intestinal adaptation. J Pediatr Surg, 41(4): 719-24, 2006.

Erwin CR, Jarboe MD, Sartor MA, Medvedovic M, Stringer K, Warner BW, Bates MD. Developmental characteristics of adapting mouse small intestine crypt cells. Gastroenterology, 130: 1324-1332, 2006.

Warner BW, Erwin CR. Critical roles for EGF receptor signaling during resection-induced intestinal adaptation. J Pediatr Gastroenterol Nutr, 43: S68-S73, 2006.

Sheng G, Bernabe KQ, Guo J, Warner BW. Epidermal growth factor receptor mediated proliferation of enterocytes requires p21^waf1/cip1 expression. Gastroenterology, 131: 153-164, 2006.

Warner BB, Ryan AL, Seeger K, Leonard AC, Erwin C, Warner BW. Ontogeny of salivary epidermal growth factor and necrotizing enterocolitis. J Pediatr, 150: 358-363, 2007.

Taylor JA, Bernabe KQ, Guo J, Warner BW. Epidermal growth faculty receptor-directed enterocyte proliferation does not induce Wnt pathway transcription. J Pediatr Surg. 42: 981-986, 2007.

Sheng G, Guo J, Warner BW. Epidermal growth factor receptor signaling modulates apoptosis via p38α MAPK-dependent activation of Bax in intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol, 293: G599-G606, 2007.

Stehr W, Bernabe KQ, Bernal NP, Erwin CR, Warner BW. STAT1 is an inhibitor of adaptation after massive small bowel resection. J Pediatr Surg, In Press.

Bernabe KQ, Bernal NP, Stehr W, Erwin CR, Warner BW. Resection induced intestinal adapation and the role of enteric smooth muscle: A cause or effect? J Pediatr Surg, In Press.