Jun Guo, PhD

Jun Guo, PhD

Associate Professor of Surgery

Expertise

Specialty Areas

  • Pediatric surgery
  • Gastrointestinal surgery
Education

Degrees

Medical School: Fudan University, Shanghai, China
Master of Science: Histology and Embryology, Dalian Medical University, Dalian, People’s Republic of China

Research

Areas of Research Interest

  • Translational medicine
  • Research design
  • Immunoassays
  • Immunotherapy
  • Mucosal immunology
  • Biochemistry
  • Molecular biology

Innovations

  1. Identified phosphatidylinositol 4-kinase type II α as the phosphatidylinositol 4-kinase associated with synaptic vesicles. During my post-doctoral training with Dr. Pietro De Camilli at Yale University, I identified a phosphatidylinositol kinase that is critical for generating PI(4)P which is a precursor of other important phosphoinositide.
    1. Salazar G, Craige B, Wainer BH, Guo J, De Camilli P, Faundez V. Phosphatidylinositol-4-kinase type II alpha is a component of adaptor protein-3-derived vesicles. Mol Biol Cell. 2005 Aug;16(8):3692-704. PubMed PMID: 15944223; PubMed Central PMCID: PMC1182308.
    1. Guo J, Wenk MR, Pellegrini L, Onofri F, Benfenati F, De Camilli P. Phosphatidylinositol 4-kinase type IIalpha is responsible for the phosphatidylinositol 4-kinase activity associated with synaptic vesicles. Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3995-4000. PubMed PMID: 12646710; PubMed Central PMCID: PMC153036.
  2. Identified Rb protein as the master regulator of enterocytes proliferation and differentiation. We found that Rb deletion in enterocytes mimic adaptation phenotype. Furthermore, Rb expression manipulation will further regulate small intestine adaptation response. These work then lead to the finding of IGF2 and CD36 as the downstream factors from Rb to modify intestinal adaptation post massive small bowel resection.
    1. Choi PM, Sun RC, Sommovilla J, Diaz-Miron J, Guo J, Erwin CR, Warner BW. IGF-2 is necessary for retinoblastoma-mediated enhanced adaptation after small-bowel resection. J Gastrointest Surg. 2014 Nov;18(11):1887-93. PubMed PMID: 25002022; PubMed Central PMCID: PMC4201888.
    1. Choi PM, Guo J, Erwin CR, Wandu WS, Leinicke JA, Xie Y, Davidson NO, Warner BW. Disruption of retinoblastoma protein expression in the intestinal epithelium impairs lipid absorption. Am J Physiol Gastrointest Liver Physiol. 2014 May 15;306(10):G909-15. PubMed PMID: 24742992; PubMed Central PMCID: PMC4024723.
    1. c.     Leinicke JA, Longshore S, Wakeman D, Guo J, Warner BW. Regulation of retinoblastoma protein (Rb) by p21 is critical for adaptation to massive small bowel resection. J Gastrointest Surg. 2012 Jan;16(1):148-55; discussion 155. PubMed PMID: 22042567; PubMed Central PMCID: PMC3779625.
    1. Guo J, Longshore S, Nair R, Warner BW. Retinoblastoma protein (pRb), but not p107 or p130, is required for maintenance of enterocyte quiescence and differentiation in small intestine. J Biol Chem. 2009 Jan 2;284(1):134-40. PubMed PMID: 18981186; PubMed Central PMCID: PMC2610504.
  3. Identified the role of mTORC1 in small intestinal adaptation after massive small bowel resection (SBR). We discovered that systemic, but not intestinal-specific mTORC1, is important for normal adaptation responses to SBR. Although not required, forced enterocyte mTORC1 signaling after resection causes an enhanced adaptive response.
    1. Barron L, Sun RC, Aladegbami B, Erwin CR, Warner BW, Guo J. Intestinal Epithelial-Specific mTORC1 Activation Enhances Intestinal Adaptation After Small Bowel Resection. Cell Mol Gastroenterol Hepatol. 2016 Dec 10;3(2):231-244. PubMed PMID: 28275690; PubMed Central PMCID: PMC5331783.

Financial Disclosures

Commbio Therapeutics

Publications

1. Guo J, Wu Y, Zhang W, Zhao J, Devi LA, Pei G, Ma L. Identification of G protein-coupled receptor kinase 2 phosphorylation sites responsible for agonist-stimulated delta-opioid receptor phosphorylation.  Mol Pharmacol. 2000 Nov; 58(5):1050-6.

2. Zhou TH, Ling K, Guo J, Zhou H, Wu YL, Jing Q, Ma L, Pei G. Identification of a human brain- specific isoform of mammalian STE20-like kinase 3 that Is regulated by cAMP-dependent protein kinase. J Biol Chem. 2000 Jan 28; 275(4):2513-9.

3. Xiang B, Yu GH, Guo J, Chen L, Hu W, Pei G, Ma L. Heterologous Activation of Protein Kinase C Stimulates Phosphorylation of delta-Opioid Receptor At Serine 344 and This Results in beta-Arrestin- and Clathrin-Mediated Receptor Internalization. J Biol Chem. 2001 Feb.16; 276(7): 4709-4716

4. Cen B, Yu Q, Guo J, Wu Y, Ling K, Cheng Z, Ma L, Pei G. Direct binding of beta-arrestins to two distinct intracellular domains of the delta opioid receptor. J Neurochem. 2001 Mar; 76 (6):1887-94.

5.  Ort T, Voronov S, Guo J, Zawalich K, Froehner SC, Zawalich W, Solimena M. Dephosphorylation of beta2 syntrophin and Ca2+/mu-calpain-mediated cleavage of ICA512 upon stimulation of insulin secretion. EMBO J. 2001 Aug 1; 20(15):4013-23

6.  Gilbert Di Paolo, Lorenzo Pellegrini, Kresimir Letinic,Gianluca Cestra, Roberto Zoncu, Sergei Voronov, Sunghoe Chang, Jun Guo, Markus Wenk and Pietro De Camilli. Recruitment and regulation of PIP kinase type1γ by the FERM domain of talin. Nature 2002 Nov 07; 420:85-89

7.  Guo J, Wenk MR, Pellegrini L, Onofri F, Benfenati F, De Camilli P. Phosphatidylinositol 4-kinase type IIalpha is responsible for the phosphatidylinositol 4-kinase activity associated with synaptic vesicles. Proc Natl Acad Sci U S A 2003 Apr 1; 100(7):3995-4000

8.  Lacas-Gervais S, Guo J, Strenzke N, Scarfone E, Kolpe M, Jahkel M, De Camilli P, Moser T, Rasband MN,  Solimena M. BetaIVSigma1 spectrin stabilizes the nodes of Ranvier and axon initial segments. J Cell Biol.  2004 Sep 27; 166(7):983-90

9.  Salazar G, Craige B, Wainer BH, Guo J, De Camilli P, Faundez V. Phosphatidylinositol-4-Kinase Type II Alpha Is a Component of AP-3-derived Vesicles. Mol Biol Cell. 2005 Aug;16(8):3692-704

10. Guo J, Sheng G and Warner BW. EGF induced rapid Rb phosphorylation at Ser780 and Ser795 is mediated by ERK1/2 in small intestine epithelial cells. J Biol Chem. 2005; 280(43):35992-8.

11. Stehr W, Bernal NP, Erwin CR, Bernabe KQ, Guo J, Warner BW. Roles for p21waf1/cip1 and p27kip1 during the adaptation response to massive intestinal resection. Am J Physiol Gastrointest Liver Physiol. 290(5):G933-41, 2006

12. Sheng G, Bernabe KQ, Guo J, Warner BW: Epidermal growth factor receptor mediated proliferation of enterocytes requires p21waf1/cip1 expression. Gastroenterology, 2006 Jul;131(1):153-64.

13. Taylor JA, Bernabe KQ, Guo J, Warner BW. Epidermal growth factor receptor-directed enterocyte proliferation does not induce Wnt pathway transcription. J Pediatr Surg. 2007 Jun;42(6):981-6

14. Sheng G, Guo J, Warner BW. Epidermal growth factor receptor signaling modulates apoptosis via p38alpha MAPK-dependent activation of Bax in intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2007 Sep;293(3):G599-606. 

15. Martin CA, Bernabe KQ, Taylor JA, Nair R, Paul RJ, Guo J, Erwin CR, Warner BW. Resection-induced intestinal adaptation and the role of enteric smooth muscle. J Pediatr Surg. 2008 Jun;43(6):1011-7.

16. Taylor JA, Martin CA, Nair R, Guo J, Erwin CR, Warner BW. Lessons learned: optimization of a murine small bowel resection model. J Pediatr Surg. 2008 Jun;43(6):1018-24.

17. Guo J, Longshore S, Nair R, Warner BW. pRb, but not p107 or p130 is required for maintenance of enterocyte quiescence and differentiation in small intestine. J Biol Chem. 2009 Jan 2;284(1):134-40.

18. Longshore SW, Nair R, Perrone EE, Erwin CR, Guo J, Warner BW. p21(waf1/cip1) deficiency does not perturb the intestinal crypt stem cell population after massive small bowel resection.J Pediatr Surg.  2009 Jun;44(6):1065-71

19. Martin CA, Perrone EE, Longshore SW, Toste P, Bitter K, Nair R, Guo J, Erwin CR, Warner BW. Intestinal resection induces angiogenesis within adapting intestinal villi. J Pediatr Surg. 2009 Jun;44(6):1077-82

20. McMellen ME, Wakeman D, Erwin CR, Guo J, Warner BW. Epidermal growth factor receptor signaling modulates chemokine (CXC) ligand 5 expression and is associated with villus angiogenesis after small bowel resection. Surgery.  2010 Aug;148(2):364-70.

21. Wakeman D, Longshore SW, McMellen ME, Santos JA, Guo J, Erwin CR, Warner BW. Extent of small bowel resection does not influence the magnitude of intestinal adaptation in the mouse. J Pediatr Surg. 2010 Jun;45(6):1274-9.

22. Leinicke JA, Longshore S, Wakeman D, Guo J, Warner BW. Regulation of retinoblastoma protein (Rb) by p21 is critical for adaptation to massive small bowel resection. J Gastrointest Surg. 2012 Jan;16(1):148-55

23. Wakeman D, Guo J, Santos JA, Wandu WS, Schneider JE, McMellen ME, Leinicke JA, Erwin CR, Warner BW. p38 MAPK regulates Bax activity and apoptosis in enterocytes at baseline and after intestinal resection. Am J Physiol Gastrointest Liver Physiol. 2012 May;302(9):G997-G1005.

24. Hitch MC, Leinicke JA, Wakeman D, Guo J, Erwin CR, Rowland KJ, Merrick EC, Heuckeroth RO, Warner BW. Ret heterozygous mice have enhanced intestinal adaptation after massive small bowel resection. Am J Physiol Gastrointest  Liver Physiol. 2012 May;302(10):G1143-50.

25. Wakeman D, Schneider JE, Liu J, Wandu WS, Erwin CR, Guo J, Stappenbeck TS, Warner BW. Deletion of p38-alpha mitogen-activated protein kinase within the intestinal epithelium promotes colon tumorigenesis. Surgery. 2012 Aug;152(2):286-93.

26. Choi P, Guo J, Erwin CR, Warner BW. IGF-2 mediates intestinal mucosal hyperplasia in retinoblastoma protein (Rb)-deficient mice. J Pediatr Surg. 2013 Jun;48(6):1340-7

27. Choi PM, Sun RC, Guo J, Erwin CR, Warner BW. High-Fat Diet Enhances Villus Growth During the Adaptation Response to Massive Proximal Small Bowel Resection. J Gastrointest Surg. 2014 Feb;18(2):286-94. doi: 10.1007/s11605-013-2338-7.

28. Diaz-Miron J, Sun R, Choi P, Sommovilla J, Guo J, Erwin CR, Mei J, Scott Worthen G, Warner BW. The effect of impaired angiogenesis on intestinal function following massive small bowel resection. J Pediatr Surg. 2015 Jun;50(6):948-53. PubMed PMID: 25818317; PubMed Central PMCID: PMC4439276.

29. Sun RC, Choi PM, Diaz-Miron J, Sommovilla J, Guo J, Erwin CR, Warner BW. High-protein diet improves postoperative weight gain after massive small-bowel resection. J Gastrointest Surg. 2015 Mar;19(3):451-7. PMID: 2551908

30. Sun RC, Diaz-Miron JL, Choi PM, Sommovilla J, Guo J, Erwin CR, Warner BW. Both epidermal growth factor and insulin-like growth factor receptors are dispensable for structural intestinal adaptation. J Pediatr Surg. 2015 Jun;50(6):943-7. PMID:25818318

31. He Y, Schmidt MA, Erwin C, Guo J, Sun R, Pendarvis K, Warner BW, Herman EM. Transgenic Soybean Production of Bioactive Human Epidermal Growth Factor (EGF). PLoS One. 2016 Jun 17;11(6). PMID: 27314851

32. Barron LK, Gayer CP, Roberts A, Golden JM, Aladegbami BG, Guo J, Erwin CR, Warner BW. Liver steatosis induced by small bowel resection is prevented by oral vancomycin. Surgery. 2016 Dec;160(6):1485-1495. PMID:27592213

33. Lauren Barron, Raphael C. Sun, Bola Aladegbami, Christopher R. Erwin, Brad W. Warner, Jun Guo. Intestinal Epithelial-Specific mTORC1 Activation Enhances Intestinal Adaptation After Small-Bowel Resection. Cell Mol Gastroenterol Hepatol. 2016 Dec 10;3(2):231-244. PMID:28275690

34. Sun RC, Choi PM, Diaz-Miron JL, Sommovilla J, Guo J, Erwin CR, Warner BW. Epithelial IGF1R is dispensable for IGF2 mediated enhanced intestinal adaptation in retinoblastoma-deficient mice. J Pediatr Surg. 2017 Jun;52(6):1026-1030

35. Barron LK, Bao JW, Aladegbami BG, Colasanti JJ, Guo J, Erwin CR, Warner BW. Toll-like receptor 4 is critical for the development of resection-associated hepatic steatosis. J Pediatr Surg. 2017 Jun;52(6):1014-1019

36. Bola Aladegbami, Lauren Barron, James Bao, Jason Colasanti, Christopher R. Erwin, Brad W. Warner, and Jun Guo. Epithelial cell specific Raptor is required for initiation of type 2 mucosal immunity in small intestine. Sci Rep. 2017 Jul 17;7(1):5580

37. Onufer EJ, Tay S, Barron LK, Courtney CM, Warner BW, Guo J.  Intestinal epithelial cell-specific Raptor is essential for high fat diet-induced weight gain in mice. Biochem Biophys Res Commun. 2018 Nov 10;505(4):1174-1179. PMID: 30318117

38. Seiler KM, Waye SE, Kong W, Kamimoto K, Bajinting A, Goo WH, Onufer EJ, Courtney C, Guo J, Warner BW, Morris SA. Single-Cell Analysis Reveals Regional Reprogramming During Adaptation to Massive Small Bowel Resection in Mice. Cell Mol Gastroenterol Hepatol. 2019;8(3):407-426. PMID: 31195149

39. Seiler KM, Bajinting A, Alvarado DM, Traore MA, Binkley MM, Goo WH, Lanik WE, Ou J, Ismail U, Iticovici M, King CR, VanDussen KL, Swietlicki EA, Gazit V, Guo J, Luke CJ, Stappenbeck T, Ciorba MA, George SC, Meacham JM, Rubin DC, Good M, Warner BW. Patient-derived small intestinal myofibroblasts direct perfused, physiologically responsive capillary development in a microfluidic Gut-on-a-Chip Model. Sci Rep. 2020 Mar 2;10(1):3842. PMID: 32123209

40. Onufer EJ, Aladegbami B, Imai T, Seiler K, Bajinting A, Courtney C, Sutton S, Bustos A, Yao J, Yeh CH, Sescleifer A, Wang LV, Guo J, Warner BW. EGFR in enterocytes & endothelium and HIF1α in enterocytes are dispensable for massive small bowel resection induced angiogenesis. PLoS One. 2020 Sep 15;15(9):e0236964. PMID: 32931498

41. Courtney CM, Shyr ZA, Yan Z, Onufer EJ, Steinberger AE, Tecos ME, Barron LK, Guo J, Remedi MS, Warner BW. Alterations in pancreatic islet cell function in response to small bowel resection. Am J Physiol Gastrointest Liver Physiol. 2020 Jul 1;319(1):G36-G42. PMID: 32463335

42. Seiler KM, Goo WH, Zhang Q, Courtney C, Bajinting A, Guo J, Warner BW. Adaptation of extracellular matrix to massive small bowel resection in mice. J Pediatr Surg. 2020 Jun;55(6):1107-1112. PMID: 32164986 

43. Tecos ME, Steinberger AE, Guo J, Warner BW. Distal Small Bowel Resection Yields Enhanced Intestinal and Colonic Adaptation. J Surg Res. 2022 May;273:100-109. PMID: 35033819

44. Steinberger AE, Tecos ME, Phelps HM, Rubin DC, Davidson NO, Guo J, Warner BW. A novel maladaptive unfolded protein response as a mechanism for small bowel resection-induced liver injury. Am J Physiol Gastrointest Liver Physiol. 2022 Sep 1;323(3):G165-G176. PMID: 35727920

Contact

To contact Dr. Guo, please call 314-747-0344.